c Kit (KIT) pTyr719 Rabbit Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA |
Recommended Dilution | ELISA: The antibody (approx. 1:64,000 diluted) can detect the above Tyr 719 phosphorylated c-kit peptide coated on ELISA plate. |
Reactivities | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | A synthetic peptide derived from tyrosine 719 phosphorylated c-kit, conjugated with KLH |
Specificity | This antibody reacts with Mouse, Human and other species with consensus c-kit peptide (SDSTNEYpMDMKPG). |
Formulation | 0.01M PBS, pH 7.4 State: Azide Free State: Lyophilized Ig fraction |
Reconstitution Method | Restore with Double distilled water to adjust the final concentration to 1.00mg/ml. |
Purification | Protein G affinity |
Conjugation | Unconjugated |
Storage | Store lyophilized at 2-8°C for 6 month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | c-kit, also known as CD117, is a receptor tyrosine kinase expressed at the surface of mast cells and certain types of stem cells. c-kit is activated and autophosphorylated upon binding with stem cell factor. Activated c-kit phosphorylates downstream SH2- domain-containing signalling molecules such as PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL. The binding is impaired when c-kit is mutated at tyrosine719. c-kit with phosphorylated tyrosine 719 binds to the p85 subunit of PI3 kinase in vitro and in vivo. C-kit is encoded by KIT gene and the gene is frequently over-expressed in gastrointestinal stromal tumor, myelogenous leukemia, and testicular germ cell tumor. Mutational loci of the gene are implicated with responsiveness to cancer drug imatinib. |
Synonyms | SCFR, KIT |
Reference Data |
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