Tnfsf10 Rabbit Polyclonal Antibody

CAT#: AP31097BT-S

Tnfsf10 rabbit polyclonal antibody, Biotin


USD 260.00

2 Weeks*

Size
    • 25 ug

Product Images

Other products for "Tnfsf10"

Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Direct ELISA: To detect Mouse TRAIL by Direct ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse TRAIL.
Sandwich ELISA: To detect Mouse TRAIL by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Purified Anti-Mouse TRAIL (Cat.-No AP31097BT-N or AP31097BT-S) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse TRAIL.
Western Blot: To detect Mouse TRAIL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse TRAIL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Mouse
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) recombinant Human soluble TRAIL Receptor-2.
Specificity Recognizes Mouse TRAIL. Other species not tested.
Formulation PBS, pH 7.2 without preservatives
Label: Biotin
State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography employing an immobilized Mouse TRAIL matrix
Conjugation Biotin
Gene Name Mus musculus tumor necrosis factor (ligand) superfamily, member 10 (Tnfsf10)
Background Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently. Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB.
Synonyms Apo-2 ligand, TNFSF10, APO2L, Apo-2L
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.