Tnfsf10 Rabbit Polyclonal Antibody

CAT#: AP31097PU-N

Tnfsf10 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot (Unreduced) using CD253 / TRAIL Antibody Cat.-No AP31097PU

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  Western Blot (Reduced) using CD253 / TRAIL Antibody Cat.-No AP31097PU

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  Sandwich ELISA using CD253 / TRAIL Antibody Cat.-No AP31097PU

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect Murine TRAIL by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Mouse TRAIL (Cat.-No AP31097BT-N or AP31097BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse TRAIL.
  Western Blot: To detect Mouse TRAIL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse TRAIL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure recombinant Murine TRAIL.
• Specificity: Recognizes TRAIL.
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing an immobilized Murine TRAIL matrix
• Gene Name: Mus musculus tumor necrosis factor (ligand) superfamily, member 10 (Tnfsf10)
• Database Link:
  Entrez Gene 22035 Mouse
• Background: TRAIL is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic Death domain which activates the cell’s apoptotic machinery.
• Synonyms: Apo-2 ligand, TNFSF10, APO2L, Apo-2L