STAT1 pSer727 Rabbit Polyclonal Antibody

CAT#: AP31321PU-N

STAT1 pSer727 rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of extracts from 293 cells, 3T3 cells treated with UV (15mins) and Jurkat cells treated with ETO (25uM, 24hours), using STAT1 (Phospho-Ser727) Antibody. The lane on the right is treated with the synthesized peptide.

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  Human Intestine, Ganglion Cells: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Immunohistochemistry on Paraffin Sections: 1/100.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from human STAT1 around the phosphorylation site of Ser727.
  Epitope: pSer727.
• Specificity: This antibody detects endogenous levels of STAT1 only when phosphorylated at Serine727.
• Formulation: PBS (without Mg2+, Ca2+), pH 7.4 containing 150 mM Sodium Chloride, 0.02% Sodium Azide and 50% Glycerol.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens signal transducer and activator of transcription 1 (STAT1), transcript variant alpha
• Database Link:
  Entrez Gene 20846 MouseEntrez Gene 25124 RatEntrez Gene 6772 Human
• Background: STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kD STAT1a and the 84 kD STAT1b which are encoded by the same gene with splicing variant.
• Synonyms: DKFZp686B04100; ISGF-3; OTTHUMP00000205845; STAT91

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chemokine signaling pathway, Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer, Toll-like receptor signaling pathway