Bovine Serum Proteins Sheep Polyclonal Antibody

CAT#: AP31473SU-N

Bovine Serum Proteins sheep polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony) to identify the serum protein pattern, or the presence or absence of an individual component. To evaluate the purity of an isolated serum protein including immunoglobulins. Since immunoprecipitation depends on a correct antigen/antibody concentration ratio (zone of equivalence) in the gel medium, the protein analysis by immunoelectrophoresis of serum or any other biological fluid or protein fraction should include different proportions of the reactants. It is not possible to obtain an optimal protein pattern in a single analysis. The electroendosmosis effect of different types of agar on proteins with a different net charge can be used to optimize the resolution power of the test system. Agar contains sufficient positively charged ions to optimize the resolution of the proteins in the beta-gamma regions, while the alpha regions will become more dense. Highly purified agar with low electroendosmosis favours the resolution of the proteins in the alpha regions, while the major components in the betagamma region can still be identified.
  Directions for use:
  In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum.
  In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
• Reactivities: Bovine
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Pooled whole bovine serum and partly purified serum fractions. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: In immunoelectrophoresis against pooled serum and enriched serum proteins fractions precipitation can be observed of not less than 11 individual proteins components.
  Cross reactivity: Inter-species cross reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross reactivity. Consequently it is not species-specific.
• Formulation: No preservative added - No foreign proteins added
  State: Serum
  State: Delipidated, heat inactivated, lyophilized, stable whole antiserum
• Reconstitution Method: Restore with 1 ml sterile distilled water