Human Lambda Light Chain (free and bound) Goat Polyclonal Antibody

CAT#: AP31534AF-N

Human Lambda Light Chain (free and bound) goat polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, WB
• Recommended Dilution: This IgG preparation is intended for use in precipitating (gel diffusion) and nonprecipitating antibody-binding immunoassays (indirect immunofluorescence, enzyme immunoassays for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the lambda type as well as free light chains or Bence Jones proteins of the lambda type in serum or other body fluids; to prepare an insoluble immunoaffinity adsorbent or a solid phase catching antibody reagent by coupling to an artificial carrier; for labeling with a marker of the user’s own choice. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
  Working dilutions:
  For histochemical and cytochemical use are usually between 1/50 and 1/250.
  In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/2000.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A pool of purified Bence Jones lambda proteins isolated from human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the lambda type, Bence Jones proteins as well as free light chains of the lambda type. This antiserum does not react with any other protein of human serum or plasma.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: PBS, pH 7.2
  No preservative added, as it may interfere with the antibody activity. No foreign proteins added.
  State: Azide Free
  State: Lyophilised hyperimmune Ig fraction
• Reconstitution Method: Restore with 1 ml sterile distilled water.
• Concentration: 10 mg/ml
• Purification: DEAE-column Chromatography
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins.