Trf Goat Polyclonal Antibody

CAT#: AP31544SU-N

Trf goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Can be used in precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence transferrin in mouse serum or other body fluids and to determine its concentration. This antiserum has not been tested in more sensitive assays as nephelometry, ELISA or use in immunohistology, but this does not exclude such use if proper controls are included.
  Directions for use:
  In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum.
  In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified transferrin isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is restricted to transferrin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified transferrin.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.
• Formulation: No preservative added. No foreign proteins added.
  State: Serum
  State: Delipidated, heat inactivated, lyophilized, stable whole antiserum
• Reconstitution Method: Restore with 1,0 ml sterile distilled water.
• Gene Name: Mus musculus transferrin (Trf)
• Database Link:
  Entrez Gene 22041 Mouse
• Synonyms: Serotransferrin, Siderophilin
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.