Tf Rabbit Polyclonal Antibody

CAT#: AP31668PU-N

Tf rabbit polyclonal antibody, Ig Fraction

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Immunoprecipitation.
  Immunodiffusion.
  
  Ouchterlony: Results
  Test 1
  Sample Size: 10 µl
  Distance Between Wells: 5 mm
  Test time: 24 hours
  Sample tested: Rat Transferrin
  Results: Positive
  
  Test 2
  Sample Size: 10 µl
  Distance Between Wells: 5 mm
  Test time: 24 hours
  Sample tested: Normal Rat serum (neat-1/1600)
  Results: one band homogeneous with Test 1.
  
  Test 3
  Sample Size: 10 µl
  Distance Between Wells: 5 mm
  Test time: 24 hours
  Sample tested: Rat Albumin and Rat IgG
  Results: Neagtive.
• Reactivities: Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Specificity: This antibody is directed against Rat Transferrin.
• Formulation: State: Ig Fraction
  State: Liquid IgG fraction without preservatives
• Concentration: 7.8 mg IgG/ml
• Purification: This IgG fraction was isolated from a high titer antiserum by salt fractionation and DEAE ion exchange chromatography. It was titered against its immunogens, sterile filtered, and analyzed for immunoglobulin purity and protein concentration.
• Gene Name: Rattus norvegicus transferrin (Tf)
• Database Link:
  Entrez Gene 24825 Rat
• Background: Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. Iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.
  The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergens from serum.
• Synonyms: Serotransferrin, Siderophilin