Coronin 1a (CORO1A) Chicken Polyclonal Antibody

CAT#: AP31804PU-N

Coronin 1a (CORO1A) chicken polyclonal antibody, Aff - Purified

Product Images

Dissociated cell culture from a neonatal Mouse brain showing Coronin 1a (green staining) of microglial cells. These cultures were counter-stained with Rabbit antibodies against NFM (neurofilament, red staining), a neuronal marker, as well as DAPI (blue staining), a marker of nuclei. Picture courtesy of Dr. Gerry Shaw, University of Florida.

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunocytochemistry.
  Immunohistochemistry.
  Recommended Dilutions: 1/1000-1/2000 for immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
  Quality Control: Quality assurance analysis was performed using Immunohistochemistry (at a dilution of 1/2000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
• Reactivities: Human, Mouse
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide KLH conjugated corresponding to a sequence shared between the Mouse (NP_034028) and Human (NP_009005) gene products.
  After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks.
• Specificity: Recognizes Coronin 1a.
• Formulation: 10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified (filter sterilized) IgY fraction.
• Concentration: 0.1 mg/ml (based on Bradford assay readings using BSA as a standard).
• Purification: Affinity Chromatography using a peptide column.
• Gene Name: Homo sapiens coronin 1A (CORO1A), transcript variant 1
• Database Link:
  Entrez Gene 12721 MouseEntrez Gene 11151 Human
• Background: Human Coronin 1a is a 51,026 dalton protein (461 amino acids) selectively expressed in cells involved in immune surveillance throughout the body, including activated microglial cells of the CNS. It is a member of the WD40 (tryptophan-aspartate) gene family, which includes the β subunit of the trimeric G proteins as the prototype. Its function is presumably in membrane structural reorganization accompanying antigenpresentation.
• Synonyms: Coronin-like protein p57, Coronin-like protein, CORO1A, CORO1, Clipin-A, TACO