NeuN (RBFOX3) Chicken Polyclonal Antibody

CAT#: AP31812PU-N

NeuN (RBFOX3) chicken polyclonal antibody, Aff - Purified


USD 570.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Applications IF, IHC
Recommended Dilution Immunocytochemistry.
Immunohistochemistry.
Recommended Dilutions: 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
Reactivities Human, Mouse
Host Chicken
Isotype Ig
Clonality Polyclonal
Immunogen Synthetic peptide KLH conjugated corresponding to the Fox-3 gene product (also known as the Neu-N antigen), but was shared between the Human (NP_001076044) and Mouse (NP_001020102) sequences.
Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 0.1 mg/ml, and the preparation filter-sterilized
Specificity Recognizes Neu-N antigen (also known as Fox-3).
Formulation 10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
State: Aff - Purified
State: Liquid purified (filter sterilized) IgY fraction.
Concentration 0.1 mg/ml (based on Bradford assay readings using BSA as a standard).
Purification Affinity Chromatography using a peptide column.
Gene Name Homo sapiens RNA binding protein, fox-1 homolog (C. elegans) 3 (RBFOX3)
Background Human Neu-N (Fox3) is a 33,873 dalton (312 amino acid) RNA-binding protein. This protein is found in the nuclei of virtually all post-mitotic central and peripheral neurons, and has been used extensively as a general neuronal marker. In these studies, Neu-N (Fox 3) stains neuronal nuclei relatively uniformly, except in the region of the nucleolus, providing a distinctive donut (i.e., torus) shape. Since this antigen is not seen in the cytoplasm, it offers the advantage of being useful in co-immunostaining studies with rabbit and Mouse antibodies against various cytoplasmic neuronal antigens.
Synonyms Fox-1 homolog C, FOX3
Reference Data

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