NeuN (RBFOX3) Chicken Polyclonal Antibody

CAT#: AP31812PU-N

NeuN (RBFOX3) chicken polyclonal antibody, Aff - Purified

Product Images

CA1 region of a transgenic Mouse expressing the eGFP gene product under control of the Thy-1 promoter. Green is eGFP autofluorescence. Red is Cy-3 secondary antibody labeling Chicken anti-Neu-N (Fox-3) positive neuronal nuclei. Picture courtesy of Dr. Felix Eckenstein, University of Vermont.

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Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunocytochemistry.
  Immunohistochemistry.
  Recommended Dilutions: 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
• Reactivities: Human, Mouse
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide KLH conjugated corresponding to the Fox-3 gene product (also known as the Neu-N antigen), but was shared between the Human (NP_001076044) and Mouse (NP_001020102) sequences.
  Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 0.1 mg/ml, and the preparation filter-sterilized
• Specificity: Recognizes Neu-N antigen (also known as Fox-3).
• Formulation: 10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified (filter sterilized) IgY fraction.
• Concentration: 0.1 mg/ml (based on Bradford assay readings using BSA as a standard).
• Purification: Affinity Chromatography using a peptide column.
• Gene Name: Homo sapiens RNA binding protein, fox-1 homolog (C. elegans) 3 (RBFOX3)
• Database Link:
  Entrez Gene 52897 MouseEntrez Gene 146713 Human
• Background: Human Neu-N (Fox3) is a 33,873 dalton (312 amino acid) RNA-binding protein. This protein is found in the nuclei of virtually all post-mitotic central and peripheral neurons, and has been used extensively as a general neuronal marker. In these studies, Neu-N (Fox 3) stains neuronal nuclei relatively uniformly, except in the region of the nucleolus, providing a distinctive donut (i.e., torus) shape. Since this antigen is not seen in the cytoplasm, it offers the advantage of being useful in co-immunostaining studies with rabbit and Mouse antibodies against various cytoplasmic neuronal antigens.
• Synonyms: Fox-1 homolog C, FOX3