Tyrosine Hydroxylase (TH) Chicken Polyclonal Antibody

CAT#: AP31824PU-N

Tyrosine Hydroxylase (TH) chicken polyclonal antibody, Aff - Purified

Product Images

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  Tissue section through an adult Mouse brain showing TYH (red staining) in catecholaminergic neurons of the substantia nigra (pars compacta). The green staining is the autofluorescence of green fluorescent protein (GFP) in neurons in this transgenic animal.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot.
  Immunocytochemistry.
  Immunohistochemistry.
  Recommended Dilutions:
  1/2000-1/5000 for Western Blots.
  1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
  Quality Control: Both of the the antibodies was analyzed by Immunohistochemistry (at 3 µg/ml) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
• Reactivities: Human, Mouse
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Two antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (P24529) and Human (P07101) gene products.
  Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of both of these affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
• Specificity: Recognizes Tyrosine Hydroxylase (TYH).
• Formulation: 10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified (filter sterilized) IgY fraction.
• Concentration: 0.2 mg/ml. The concentrations of each of the two antibodies was 0.1 mg/ml (based on Bradford assay readings using BSA as a standard).
• Purification: Affinity Chromatography using a peptide column.
• Gene Name: Homo sapiens tyrosine hydroxylase (TH), transcript variant 2
• Database Link:
  Entrez Gene 21823 MouseEntrez Gene 7054 Human
• Background: Human TYH (EC 1.14.16.2) is a 58,523 dalton protein (528 amino acids) responsible for the enzymatic conversion of L-tyrosine to L-DOPA (dihydroxyphenylalanine). This enzyme is expressed in all catecholaminergic neurons of the CNS and PNS. In the CNS, TYH-positive neurons can be found within the substantia nigra, ventral tegmental area, locus ceruleus, and hypothalamus. In the PNS, TYH-positive neurons can be found within the sympathetic chain, pre-vertebral ganglia and the adrenal medulla.
• Synonyms: Tyrosine 3-hydroxylase, TYH

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Parkinson's disease, Tyrosine metabolism