Spasmolytic Polypeptide (TFF2) Rabbit Polyclonal Antibody

CAT#: AP31836BT-N

Spasmolytic Polypeptide (TFF2) rabbit polyclonal antibody, Biotin

Product Images

Direct ELISA using Biotin conjugated Rabbit anti-Human TFF-2 antibody

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  Sandwich ELISA using Biotin conjugated Rabbit anti-Human TTF-2 antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human TFF-2 by Direct ELISA (using 100 μl/well) a concentration of approximately 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2–0.4 ng/well of recombinant Human TFF-2.
  Sandwich ELISA: To detect Human TFF-2 by Sandwich ELISA (using 100 μl/well) a concentration of 0.25–1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human TFF-2 (Cat.-No AP31836PU-N/S) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of recombinant Human TFF-2.
  Western Blot:To detect Human TFF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human TFF-2 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure E.coli derived Recombinant Human Trefoil Factor 2
• Specificity: Recognizes TFF-2
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C for one year.
  Following reconstitution the antibody can be stored at 2-8°C for 2 weeks.
  Avoid repeated freezing and thawing.
• Gene Name: Homo sapiens trefoil factor 2 (TFF2)
• Database Link:
  Entrez Gene 7032 Human
• Background: The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF2 has been shown to inhibit gastrointestinal motility and gastric acid secretion. Recent data suggests a potential role for TFF2 in acute and chronic asthma (Nikolaidis, N.M. et al. Am. Journal Respir. Cell Mol. Biol. (2003) 4: 458-464).
• Synonyms: TFF2, Spasmolytic polypeptide, SP, Spasmolysin
• Note: Centrifuge vial prior to opening!

Reference Data

• Protein Families: Druggable Genome, Secreted Protein