Spasmolytic Polypeptide (TFF2) Rabbit Polyclonal Antibody

CAT#: AP31836BT-N

Spasmolytic Polypeptide (TFF2) rabbit polyclonal antibody, Biotin


USD 355.00

2 Weeks*

Size
    • 50 ug

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Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Direct ELISA: To detect Human TFF-2 by Direct ELISA (using 100 μl/well) a concentration of approximately 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2–0.4 ng/well of recombinant Human TFF-2.
Sandwich ELISA: To detect Human TFF-2 by Sandwich ELISA (using 100 μl/well) a concentration of 0.25–1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human TFF-2 (Cat.-No AP31836PU-N/S) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of recombinant Human TFF-2.
Western Blot:To detect Human TFF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human TFF-2 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure E.coli derived Recombinant Human Trefoil Factor 2
Specificity Recognizes TFF-2
Formulation PBS, pH 7.2
Label: Biotin
State: Lyophilized purified Ig fraction
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography
Conjugation Biotin
Storage Store the antibody prior to reconstitution at -20°C for one year.
Following reconstitution the antibody can be stored at 2-8°C for 2 weeks.
Avoid repeated freezing and thawing.
Gene Name Homo sapiens trefoil factor 2 (TFF2)
Background The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF2 has been shown to inhibit gastrointestinal motility and gastric acid secretion. Recent data suggests a potential role for TFF2 in acute and chronic asthma (Nikolaidis, N.M. et al. Am. Journal Respir. Cell Mol. Biol. (2003) 4: 458-464).
Synonyms TFF2, Spasmolytic polypeptide, SP, Spasmolysin
Note Centrifuge vial prior to opening!
Reference Data
Protein Families Druggable Genome, Secreted Protein

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.