Gelsolin (GSN) (Isoform a) Goat Polyclonal Antibody

CAT#: AP31939PU-N

Gelsolin (GSN) (Isoform a) goat polyclonal antibody, Aff - Purified

Product Images

Gelsolin antibody staining of Human Duodenum lysate at 1 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution:
  Peptide ELISA: 1/64000 (Detection limit).
  Western Blot: 1-3 µg/ml. Detects a band of Approx 100kDa in Human Colon, Duodenum, Heart and Skeletal Muscle lysates.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic Peptide (PQGRVPEARPNS-C) from the N-Terminus of the protein sequence according to NP_000168.1; NP_937895.1; NP_001121138.1.
• Specificity: This antibody is expected to recognize Isoform a (NP_000168.1) only.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 85.7 kDa (NP_000168.1).
• Database Link:
  Entrez Gene 2934 Human
• Background: Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
  Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
  Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
• Synonyms: Brevin, AGEL, GSN