AP2 alpha (AP2A1) (706-727) Goat Polyclonal Antibody

CAT#: AP32069PU-N

AP2 alpha (AP2A1) (706-727) goat polyclonal antibody, Aff - Purified

Product Images

Staining of Human Frontal Cortex lysate using AP2A1 Antibody at 0.01 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution:

  Peptide ELISA: 1/128000 (Detection Limit).
  Western blot: 0.01-0.03 μg/ml. Approx 110kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kDa according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates.
  Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.
  

• Reactivities: Bovine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_055018.2.
• Specificity:
  This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 160 Human
• Synonyms: CLAPA1, Alpha adaptin A, Alpha-1 adaptin, AP2 complex subunit alpha-1