Factor IX (F9) Rabbit Polyclonal Antibody

CAT#: AP33070SU-N

Factor IX (F9) rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Immunoprecipitation.
  Can be used in precipitating techniques as electroimmunodiffusion, immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony). To prepare an adsorbent for immunoaffinity purification of FIX. If used in more sensitive test procedures or as catching or detection antibody in solid phase immunoassays specificity controls should always be include. Plasma samples and all assay components must contain EDTA to stabilize the proteins. Normal adult plasma contains an average of 1 mg FIX per 100 ml. In a normal population this varies between 80% and 120% of the average. Newborn infants have a level of about 75% of the average adult level. Absence of FIX in plasma causes a bleeding diathesis very similar to haemophilia A in clinical manifestations and from the point of view of heredity. It is known as haemophilia B or Christmas disease. Different types have been described. The most common type demonstrates reduced FIX coagulant activity without measurable FIX relates antigen. Other types of reduced FIX antigen and coagulation activity, or reduced coagulant activity with normal antigen levels. Heterozygote carriers can be detected. Acquired depression of plasma FIX levels are seen in vitamin K deficiencies, in liver disease, or following therapy with vitamin K antagonists (oral coagulants).
  Directions for Use: In immunoelectrophoresis in agarose-plates use 2 µl human plasma or equivalent against 120 µl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.
  Precipitin titre: not less than 1/32 when tested against appropriate concentrations of the antigen in agar-block immunodiffusion titration. The amount of Factor IX precipitated by 1 ml antiserum is between 15 and 25 U. One Unit is the average amount of FIX in 1 ml normal plasma which corresponds to between 8 and 12 µg.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: For immunization purposes, FIX can be obtained in highly purified form from fresh plasma concentrates. After coagulation, FIX antigen is also present in serum. Abnormal molecular variants are defective in proteolytic activation and serine protease activity, while calcium binding is also impaired.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The defined antibody reactivity is restricted to Factor IX as tested at the level of sensitivity of immuno-precipitation techniques. A single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen. No precipitation is obtained with any other plasma protein and with haemophilia B plasma.
  Cross-reactivity: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: State: Serum
  State: Lyophilized (Delipidated, heat inactivated) stable whole serum.
  Preservative: None
• Reconstitution Method: Restore by adding 1 ml sterile distilled water
• Concentration: lot specific
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2158 Human
• Background: Factor IX is a vitamin K-dependent glycoprotein, MW 55,400, and it consists of a single polypeptide chain. It is synthesized in the liver, stable for several days in fresh blood and for many months in fresh frozen plasma. It can be prepared as a stable concentrate. Its physicochemical properties are similar to those of several other clotting factors, which makes separation particularly difficult. Factor IX is converted to FIXa by FXIa in the presence of calcium ions. Platelets possess FXI-like activity, bypassing FXII an XI. FIXa has a molecular weight of 46,500 and is composed of two chains linked by disulphide bonds.
• Synonyms: Christmas factor, PTC
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.