Erythrocytes Rabbit Polyclonal Antibody

CAT#: AP33080SU-N

Erythrocytes rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Immunoprecipitation.
  Can be used to sensitize cells in hemolytic complement assays and for lymphocytic screening procedures.
  Antibody titre: Hemolytic titre at which 50% lysis occurs following mixing of equal volumes of antiserum, Guinea Pig complement (at a dilution of 1/60) and 10⁸ RBC/ml not less than 1/3,000 when tested with Sheep Erythrocytes.
• Reactivities: Sheep
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Washed whole Erythrocytes were prepared from fresh blood and used for intravenous immunization.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Sheep Erythrocytes.
  Cross-Reactivity: Inter-species cross-reactivity is a normal feature of antibodies since different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: State: Serum
  State: Delipidated, heat inactivated lyophilized stable whole serum
  Stabilizer: None
  Preservative: None, as it may interfere with the antibody activity.
• Reconstitution Method: Restore with 1 ml sterile distilled water.
  Dilutions may be prepared by adding PBS, pH 7.2
• Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
• Note: Adsorption: Not required.