Amyloid beta A4 protein / APP (433-452) Chicken Polyclonal Antibody

CAT#: AP33337PU-N

Amyloid beta A4 protein / APP (433-452) chicken polyclonal antibody, Aff - Purified

Product Images

Amyloid Precursor Protein (APP), Peptide #3: Mouse cortical neurons in culture. The green staining is APP-immunoreactivity, using Fluorescein-labeled Goat anti-Chicken IgY (AP31795FC-N) and Rhodamine-labeled phalloidin as a counterstain. Note the APP-stain

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunocytochemistry.
  Immunohistochemistry.
  Recommended Dilutions: 1/500-1/1000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
  Quality Control: This anti-Peptide antibody mixture was analyzed by Immunohistochemistry (1/1000 dilution) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
• Reactivities: Human, Mouse, Rat
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Chickens were immunized with a Keyhole Limpet Hemocyanin (KLH) conjugate of the synthetic peptide QHF QEK VES LEQ EAA NER QQ, which corresponds to residues #433-452 of the Human gene product (NP_000475.1).
  This sequence is also conserved in the Mouse and Rat APP gene products, as well.
• Specificity: Recognizes Amyloid Precursor Protein (APP), Peptide #3.
• Formulation: Sodium Phosphate-Buffered (10 mM) isotonic (0.9%, w/v) Saline (PBS, pH 7.2)
  State: Aff - Purified
  State: Liquid purified (filter sterilized) IgY fraction
  Preservative: 0.02% (w/v) Sodium Azide
• Concentration: 0.1 mg/ml (based on absorbance at 280 nm)
• Purification: Affinity Chromatography using a peptide column.
  Preparation: After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified against the cognate peptide using an agarose column, the concentration of the eluates adjusted to 100 μg/ml, and the preparation was filter-sterilized.
• Synonyms: Alzheimer disease amyloid protein, Amyloid Precursor Protein, ABPP, APPI, PreA4, Cerebral vascular amyloid peptide, CVAP