AP2M1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP50202PU-N

AP2M1 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane) using AP2M1 Antibody (C-term). This demonstrates the AP2M1 antibody detected the AP2M1 protein (arrow).

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  AP2M1 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/100-1/500.
  Flow Cytometry: 1/10-1/50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 386-415 amino acids from the C-terminal region of Human AP2M1
• Specificity: This antibody recognizes AP2 complex subunit mu-1.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 49655 Da
• Gene Name: Homo sapiens adaptor related protein complex 2 mu 1 subunit (AP2M1), transcript variant 2
• Database Link:
  Entrez Gene 11773 MouseEntrez Gene 1173 Human
• Background: This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: CLAPM1, KIAA0109, Adaptin-mu2, AP-2 mu-2 chain

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Huntington's disease