BCL3 (Center) Rabbit Polyclonal Antibody

CAT#: AP50358PU-N

BCL3 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of BCL3 Antibody (Center) in mouse liver tissue lysates (35ug/lane). BCL3 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCL3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of BCL3 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  BCL3 Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 249~277 amino acids from the Center region of Human BCL3
• Specificity: This antibody recognizes Bcl-3.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Protein A Chromatography followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 46800 Da
• Gene Name: B-cell CLL/lymphoma 3
• Database Link:
  Entrez Gene 602 Human
  UniProt ID P20749
• Background: BCL3 is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers.
• Synonyms: BCL3, BCL4, D19S37

Reference Data

• Protein Families: Druggable Genome, Transcription Factors