Calpain 3 (CAPN3) (C-term) Rabbit Polyclonal Antibody

CAT#: AP50723PU-N

Calpain 3 (CAPN3) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of Crified Pab.

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  CAPN3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CAPN3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  CAPN3 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blot: 1/50 - 1/100.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Flow cytometric: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 610~640 amino acids from the C-terminal region of human CAPN
• Specificity: This antibody reacts to CAPN3.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 94254 Da
• Gene Name: Homo sapiens calpain 3 (CAPN3), transcript variant 1
• Database Link:
  Entrez Gene 825 Human
• Background: Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A.
• Synonyms: Calpain L3, Calpain p94, New calpain 1, CAPN3, CANP3, CANPL3, NCL1

Reference Data

• Protein Families: Druggable Genome, Protease