IBTK (Center) Rabbit Polyclonal Antibody

CAT#: AP52138PU-N

IBTK (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of IBTK / BTKI Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the IBTK antibody detected the IBTK protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue reacted with IBTK / BTKI Antibody (Center), which was peroxidase conjugated to the secondary antibody and followed by DAB staining.

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  Flow cytometric analysis of MDA-MB435 cells using IBTK / BTKI Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 589-617 amino acids from the Central region of human IBTK / BTKI
• Specificity: This antibody recognizes Human IBTK / BTKI (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens inhibitor of Bruton tyrosine kinase (IBTK), transcript variant 1
• Database Link:
  Entrez Gene 25998 Human
• Background: The protein encoded by this gene binds to Bruton's tyrosine kinase (BTK) and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription.
• Synonyms: KIAA1417
• Note: Molecular Weight: 150528 Da (isoform 130kd Da)

Reference Data

• Protein Families: Druggable Genome