IL6 (Center) Rabbit Polyclonal Antibody

CAT#: AP52199PU-N

IL6 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Interleukin-6 (IL6) Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). This demonstrates the IL6 antibody detected the IL6 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue reacted with Interleukin-6 (IL6) Antibody (Center) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Flow cytometric analysis of NCI-H460 cells using Interleukin-6 (IL6) Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 26-55 amino acids from the Central region of Human Interleukin-6
• Specificity: This antibody recognizes Human Interleukin-6 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens interleukin 6 (IL6), transcript variant 1
• Database Link:
  Entrez Gene 3569 Human
• Background: This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.
• Synonyms: IL-6, Interferon beta-2, IFNB2, B-cell stimulatory factor 2, BSF-2, CDF
• Note: Molecular Weight: 23718 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Jak-STAT signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prion diseases, Toll-like receptor signaling pathway