MAN2A2 (Center) Rabbit Polyclonal Antibody

CAT#: AP52594PU-N

MAN2A2 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of MAN2A2 Antibody (Center) in mouse Neuro-2a cell line lysates (35ug/lane). This demonstrates the MAN2A2 antibody detected the MAN2A2 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue reacted with MAN2A2 Antibody (Center), which was peroxidase conjugated to the secondary antibody and followed by DAB staining.

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  Flow cytometric analysis of Neuro-2a cells using MAN2A2 Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.

• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 766-796 amino acids from the Central region of human MAN2A2
• Specificity: This antibody recognizes Human and Mouse MAN2A2 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant 1
• Database Link:
  Entrez Gene 140481 MouseEntrez Gene 4122 Human
• Background: MAN2A2 catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
• Synonyms: Alpha-mannosidase 2x, Mana2x, Alpha-mannosidase IIx, Man IIx, Mannosidase alpha class 2A member 2, Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
• Note: Molecular Weight: 129282 Da

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis