Germinal Center Kinase (MAP4K2) (Center) Rabbit Polyclonal Antibody

CAT#: AP52600PU-N

Germinal Center Kinase (MAP4K2) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of MAP4K2 Antibody (Center) in Jurkat cell line lysates (35ug/lane). This demonstrates the MAP4K2 antibody detected the MAP4K2 protein (arrow).

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  Flow cytometric analysis of Jurkat cells using MAP4K2 Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 320-349 amino acids from the Central region of human MAP4K2
• Specificity: This antibody recognizes Human MAP4K2 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens mitogen-activated protein kinase kinase kinase kinase 2 (MAP4K2), transcript variant 1
• Database Link:
  Entrez Gene 5871 Human
• Background: The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1.
• Synonyms: MAPK, MEK kinase kinase 2, MEKKK 2, RAB8IP
• Note: Molecular Weight: 91556 Da

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway