MAP3K4 (Center) Rabbit Polyclonal Antibody

CAT#: AP52665PU-N

MAP3K4 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of MEKK4 Antibody (Center) in human normal Uterus cell line lysates (35ug/lane). This demonstrates the MEKK4 antibody detected the MEKK4 protein (arrow).

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  Formalin fixed and paraffin embedded human heart stained with MEKK4 Antibody (Center) tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1065-1097 amino acids from the Central region of human MEKK4
• Specificity: This antibody reacts to MEKK4.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 181685 Da
• Gene Name: Homo sapiens mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant 1
• Database Link:
  Entrez Gene 4216 Human
• Background: The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described.
• Synonyms: MAPK/ERK kinase kinase 4, MAP three kinase 1, MAPKKK4, MEKK4, MTK1
• Note:
  

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: GnRH signaling pathway, MAPK signaling pathway