MITF (N-term) Rabbit Polyclonal Antibody

CAT#: AP52701PU-N

MITF (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of MITF Antibody (N-term) in K562 cell line lysates (35ug/lane). This demonstrates the MITF antibody detected the MITF protein (arrow).

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  Confocal immunofluorescent analysis of MITF Antibody (N-term) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of K562 cells using MITF Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 8-36 amino acids from the N-terminal region of Human MITF.
• Specificity: This antibody recognizes Human MITF (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens microphthalmia-associated transcription factor (MITF), transcript variant 4
• Database Link:
  Entrez Gene 4286 Human
• Background: This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Synonyms: Microphthalmia-associated transcription factor, Mi-protein
• Note: Molecular Weight: 58795 Da

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Melanogenesis, Melanoma, Pathways in cancer