PCCA (Center) Rabbit Polyclonal Antibody

CAT#: AP53192PU-N

PCCA (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

PCCA Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PCCA antibody detected the PCCA protein (arrow).

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  PCCA Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCCA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 336-365 amino acids from the Central region of human PCCA
• Specificity: This antibody reacts to PCCA.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 80059 Da
• Gene Name: Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant 1
• Database Link:
  Entrez Gene 5095 Human
• Background: The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: PCCase subunit alpha

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation