PCDHGA1 (Center) Rabbit Polyclonal Antibody

CAT#: AP53213PU-N

PCDHGA1 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PCDHGA1 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the PCDHGA1 antibody detected the PCDHGA1 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with followed by peroxidase conjugation of the secondary antibody and DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human PCDHGA1
• Specificity: This antibody recognizes Human PCDHGA1 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens protocadherin gamma subfamily A, 1 (PCDHGA1), transcript variant 1
• Database Link:
  Entrez Gene 56114 Human
• Background: This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
• Synonyms: Protocadherin gamma-A1, PCDH-gamma-A1
• Note: Molecular Weight: 90kd isoform; 101226 Da

Reference Data

• Protein Families: Transmembrane