PIGA (C-term) Rabbit Polyclonal Antibody

CAT#: AP53294PU-N

PIGA (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in MDA-MB435 cell line lysates (35ug/lane) using PIGA antibody. This demonstrates the PIGA antibody detected the PIGA protein (arrow).

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  Immunohistochemistry analysis in human brain tissue using PIGA antibody. (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PIGA antibody for IHC. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  Immunohistochemistry on Paraffin sections: 1/10-1/50.
  Western blot: 1/1000.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region of human PIGA.
• Specificity: This antibody recognizes PIGA at C-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens phosphatidylinositol glycan anchor biosynthesis class A (PIGA), transcript variant 1
• Database Link:
  Entrez Gene 5277 Human
• Background: The PIGA gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12.
• Synonyms: PIG-A
• Note: Molecular Weight: 54127 Da

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways