POLR1B (N-term) Rabbit Polyclonal Antibody

CAT#: AP53382PU-N

POLR1B (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of POLR1B Antibody (N-term) in Hela cell line lysates (35ug/lane). This demonstrates the POLR1B antibody detected the POLR1B protein (arrow).

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  Formalin fixed and paraffin embedded human stomach tissue reacted with POLR1B Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Flow cytometric analysis of Hela cells using POLR1B Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: conjugated synthetic peptide between 88-117 amino acids from the N-terminal region of human POLR1B
• Specificity: This antibody recognizes Human POLR1B (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinitty Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 2
• Database Link:
  Entrez Gene 84172 Human
• Background: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).
• Synonyms: RNA polymerase I subunit 2, RPA135
• Note: Molecular Weight: 128229 Da

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase