LOC105369264 (Center) Rabbit Polyclonal Antibody

CAT#: AP53510PU-N

LOC105369264 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in K562 cell line lysates (35ug/lane) using PTPN20 antibody. This demonstrates the PTPN20A antibody detected the PTPN20A protein (arrow).

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  Immunohistochemistry analysis in human stomach tissue (Formalin-fixed, Paraffin-embedded) using PTPN20 antibody., followed by peroxidase conjugated secondary antibody and DAB staining.This data demonstrates the use of PTPN20 antibody for IHC. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram) using PTPN20 antibody., followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/10-1/50.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the central region (between 185-215aa) of human PTPN20A.
• Specificity: This antibody recognizes PTPN20.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens protein tyrosine phosphatase, non-receptor type 20 (PTPN20), transcript variant 1
• Database Link:
  Entrez Gene 26095 HumanEntrez Gene 105369264 Human
• Background: The product of the PTPN20A (and PTPN20B) gene belongs to the family of classical tyrosine-specific protein tyrosine phosphatases. Many protein tyrosine phosphatases have been shown to regulate fundamental cellular processes and several are mutated in human diseases. Chromosome 10q contains a segmental duplication resulting in multiple copies of the protein tyrosine phosphatase, non-receptor type 20 gene. The two nearly identical copies are designated as PTPN20A and PTPN20B. A third copy is only partially duplicated and contains a pseudogene, designated as PTPN20C. This gene encodes the more telomeric copy, PTPN20B. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
• Synonyms: PTPN20A, PTPN20B, RP11-142I17.1
• Note: Molecular Weight: 26kd Isoform 6. 48423 Da

Reference Data

• Protein Families: Druggable Genome, Phosphatase