SCEL (Center) Rabbit Polyclonal Antibody

CAT#: AP53810PU-N

SCEL (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of SCEL Antibody (Center) in NCI-H292, A549 cell line lysates (35ug/lane). This demonstrates the SCEL antibody detected the SCEL protein (arrow).

• 

  Flow Cytometric analysis of A549 cells using SCEL Antibody (Center) (Right histogram) compared to a negative control cell (Left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 247-277 amino acids from the Central region of Human SCEL
• Specificity: Recognizes SCEL (Center).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide Affinity purification
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens sciellin (SCEL), transcript variant 2
• Database Link:
  Entrez Gene 8796 Human
• Background: The protein encoded by this gene is a precursor to the cornified envelope of terminally differentiated keratinocytes. This protein localizes to the periphery of cells and may function in the assembly or regulation of proteins in the cornified envelope. Transcript variants encoding different isoforms exist. A transcript variant utilizing an alternative polyA signal has been described in the literature, but its full-length nature has not been determined.
• Synonyms: FLJ21667; MGC22531; sciellin
• Note: Molecular Weight: 77552 Da