TGIF (TGIF1) (Center) Rabbit Polyclonal Antibody

CAT#: AP54235PU-N

TGIF (TGIF1) (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of TGIF1 (arrow) using rabbit polyclonal TGIF1 Antibody (Center L223). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene.

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  TGIF1 Antibody (Center L223) western blot analysis in mouse bladder tissue lysates (35 ug/lane). This demonstrates the TGIF1 antibody detected the TGIF1 protein (arrow).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:1;000.
  Western blot: 1:50~100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 215-245 amino acids from the Central region of human TGIF1
• Specificity: This antibody detects TGIF1 (Center).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens TGFB induced factor homeobox 1 (TGIF1), transcript variant 4
• Database Link:
  Entrez Gene 21815 MouseEntrez Gene 7050 Human
• Background: The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and eight variants, encoding four distinct isoforms, are described.
• Synonyms: TGIF
• Note: Molecular Weight: 43013 Da

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors