TRADD (Center) Rabbit Polyclonal Antibody

CAT#: AP54350PU-N

TRADD (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

TRADD Antibody (Center) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the TRADD antibody detected the TRADD protein (arrow).

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  TRADD Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TRADD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of TRADD Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

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  TRADD Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1:1;000.
  Western blot: 1:100~500.
  Immunohistochemistry on paraffin sections: 1:50~100.
  Flow cytometry: 1:10~50.
  Immunofluorescence: 1:10~50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 133-159 amino acids from the Central region of human TRADD
• Specificity: This antibody detects TRADD (Center).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: TNFRSF1A associated via death domain
• Database Link:
  Entrez Gene 8717 Human
  UniProt ID Q15628
• Background: The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. [provided by RefSeq].
• Synonyms: MGC11078
• Note: Molecular Weight: 34247 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Apoptosis, RIG-I-like receptor signaling pathway