Ly6c1 Rat Monoclonal Antibody [Clone ID: ER-MP20]

CAT#: BM4019F

Ly6c1 rat monoclonal antibody, clone ER-MP20, FITC


USD 675.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name ER-MP20
Applications FC, IHC
Recommended Dilution Flow Cytometry.
Suggested positive control: Monocytes.
Has been described to work in Immunohistology.
Reactivities Mouse
Host Rat
Isotype IgG2a
Clonality Monoclonal
Immunogen Mouse macrophage cell lines
Specificity This Monoclonl antibody ER-MP20 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is ideally suitable for the detection of monocytes in bone marrow samples by FACS. ER-MP20 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 (anti F4/80 antibody BM4007) is recommended. ER-MP20 also detects a wide range of endothelial cells.
Antigen Distribution on Isolated cells: In bone marrow cells the antigen is found on monoblasts and late CFU-M cells as well as on monocytes. It is also found on granulocytes and a subpopulation of lymphocytes in the peripheral blood. Granulocytic cells show a dull, and monocytic cells a bright antigen surface expression. Lymphoid cells express the antigen only very weakly. Thus, in the bone marrow three useful FACS windows can be defined for cell sorting purposes.
Antigen Distribution on Tissue Sections: The antigen is found on macrophage precursor subpopulations in the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Endothelial cells of small vessels in various organs also stain positive with ER-MP20. Activated macrophages in inflammatory tissues also express the ER-MP20-related antigen.
Formulation PBS, pH 7.2 containing 10 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a presevative
Label: FITC
State: Liquid purified Ig fraction
Label: Fluorescein Isothiocyanate
Concentration 0.1 mg/ml
Purification Affinity Chromatography
Conjugation FITC
Storage Store the antibody undiluted at 2-8°C
Stability Shelf life: One year from despatch.
Background Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes.
Synonyms Lymphocyte antigen 6C2, Ly-6C2, Ly6C2, Mouse Macrophage Marker
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.