CD68 Mouse Monoclonal Antibody [Clone ID: Ki-M7]

CAT#: BM4111F

CD68 mouse monoclonal antibody, clone Ki-M7, FITC

Product Images

Staining of human peripheral blood monocytes with Mouse anti Human CD68 antibody -FITC (BM4111F) following permeabilisation.

Specifications

Product Data

• Clone Name: Ki-M7
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl to label 10⁶ cells in 100 µl (Membrane permeabilisation is required).
  Clone KiM7 has been reported as being also suitable for use in Immunoprecipitation and Immunohistochemistry on Frozen Sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cell suspensions of Human lymph node
• Specificity: CD68 antibody (Clone Ki-M7) Cat.-No BM4111F recognizes the 110KD integral membrane glycoprotein CD68 (Macrosialin) predominantly expressed on the intracellular lysosomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes.
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: 1% BSA
  Preservative: 0.05% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
  Absorption emission: 495 nm / 528 nm
• Purification: Protein A Chromatography
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FEEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 968 Human
• Background: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
  Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukaemia studies.
• Synonyms: Gp110, Macrosialin, Macrophage marker