E. coli (O+K antigens) Rabbit Polyclonal Antibody

CAT#: BP1021B

E. coli (O+K antigens) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: Avidin and streptavidin amplification systems for ELISA and Fluorescence Microscopy.
  Has been described for previous lots to work in Western blot.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mixture of E. coli serotypes
• Specificity: Many O and K antigenic serotypes of E. coli.
  Not absorbed and does cross-react with related Enterobacteriaceae.
  Will remove E.coli proteins from recombinant preparations.
• Formulation: 0.01 M PBS, pH 7.2 containing 0.09% Sodium Azide as preservative and no stabilizers.
  Label: Biotin
  State: Liquid purified IgG fraction.
  Label: Covalently coupled with the N-Hydroxysuccinimide ester of under mild conditions to give a high degree of substitution
• Concentration: lot specific
• Purification: Protein A Chromatography.
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
• Synonyms: Escherichia coli, EHEC, EPEC, ETEC