E. coli (O+K antigens) Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | IF |
Recommended Dilution | Indirect Immunofluorescence (titer >1:1,600) assay vs serotypes O157:H7, O20, O125, O55, O111 and K12. Direct Fluorescenceimmunoassay staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC. |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Mixture of E. coli serotypes |
Specificity | Many "O" and "K" antigenic serotypes of Escherichia coli. Antiserum is not absorbed and does crossreact with other members of the Enterobacteriaciae. |
Formulation | 0.01 M PBS, pH 7.2 with 0.1% sodium azide as preservative and 10 mg/ml BSA as stabilizer. Label: FITC State: Liquid purified Ig fraction (>95% pure) Label: High purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product |
Concentration | lot specific |
Conjugation | FITC |
Storage | Store the antibody at 2-8°C under subdued light for one month or (in aliquots) at -20°C for longer. This product is phototsensitive and should be protected from light. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Synonyms | Escherichia coli, EHEC, EPEC, ETEC |
Reference Data |
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.