Collagen IV (COL4A1) Rabbit Polyclonal Antibody

CAT#: BP8018

Collagen IV (COL4A1) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: ELISA: > 1/200 gives OD > 500.
  RIA.
  Immunofluorescence (1/80).
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 1/500.
  Incubation time: 60 min. at RT or 2-8°C overnight.
  Positive Control: Porcine renals.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Porcine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified collagen type IV from porcine renals.
• Specificity: This antibody is specific for Collagen type IV (100%).
  Porcine Collagen type I, II, III, Elastin, Fibronectin and Laminin < 0.1% (in RIA at 1/1000 dilution).
• Formulation: PBS without BSA and preservatives.
  State: Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 0.5 ml distilled water.
  For further dilution use appropriate antibody diluent.
• Concentration: lot specific
• Purification: Affinity Chromatography.
• Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20 °C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens collagen type IV alpha 1 (COL4A1), transcript variant 1
• Database Link:
  Entrez Gene 1282 Human
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type IV Collagen (170-180 kDa), is a non-fibrilary network of different alpha-chains. It is typically found in basal membranes of different organs (e.g. skin, lens, lung, renals).
• Synonyms: COL4A1

Reference Data

• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer