Collagen V (COL5A1) Rabbit Polyclonal Antibody

CAT#: BP8019

Collagen V (COL5A1) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: IF: 1:40.
  IHC: 1:200 - 1:600 for paraffin sections, 60 min at RT or 2-8°C over night.
  ELISA 1:200 gives OD > 500.
  RIA.
  Positive control: chicken skin or liver.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Chicken
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified collagen type IV from chicken skin
• Specificity: Chicken collagen type V 100 %, chicken collagen type I, II, III and X < 0.1 %; chicken fibronectin < 0.1 %; human collagen type V < 0.1 %.
• Formulation: State: Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: Reconstitute with 0.5 ml distilled water, for further dilution use appropriate antibody diluent
• Concentration: lot specific
• Purification: Ion exchange chromatography
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens collagen type V alpha 1 (COL5A1), transcript variant 1
• Database Link:
  Entrez Gene 1289 Human
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Collagen type V is found as short fibrils in skin, bone and placenta. Often it is found in conjunction with other collagen types, especially type I and III.
• Synonyms: COL5A1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion