Collagen IV (COL4A1) Rabbit Polyclonal Antibody

CAT#: BP8031

Collagen IV (COL4A1) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: IF: 1:80.
  IHC: 1:500 - 1:1000 for paraffin sections, 60 min at RT or 2-8°C over night.
  ELISA.
  RIA.
  Positive control: Human skin, placenta or lens capsule.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified collagen type IV from human placenta
• Specificity: Human collagen type IV 100%;
  human collagen I, II < 0.1% ;
  human collagen III, V < 1.0%;
  human fibronectin < 0.1%;
  mouse laminin < 0.1% (RIA at 1:10000 dilution).
• Formulation: Phosphate buffered solution; no BSA and preservative added
  State: Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: Reconstitute with 0.5 ml distilled water. For further dilution use appropriate antibody diluent.
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens collagen type IV alpha 1 (COL4A1), transcript variant 1
• Database Link:
  Entrez Gene 1282 Human
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type IV Collagen (170-180 kDa), is a non-fibrilary network of different alpha-chains. It is typically found in basal membranes of different organs (e.g. skin, lens, lung, renals).
• Synonyms: COL4A1

Reference Data

• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer