Collagen V (COL5A1) Rabbit Polyclonal Antibody

CAT#: BP8032

Collagen V (COL5A1) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: Immunofluorescence: 1/40.
  Immunohistochemistry on Paraffin Sections: 1/200-1/600 for 60 min at RT or 2-8°C over night.
  ELISA.
  RIA.
  Positive Control: Human placenta, Skin.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified collagen type V from Human placenta
• Specificity: Human Collagen type V: 100% binding in RIA at 1/100 dilution.
  Cross-reactivity with
  human Collagen type I, III and IV: < 0.1%;
  Human Fibronectin: < 0.1%;
  Mouse Laminin: < 0.1%.
• Formulation: PBS
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: None
  Preservative: None
• Reconstitution Method: Restore with 0.5 ml distilled water. For further dilution use appropriate antibody diluent.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens collagen type V alpha 1 (COL5A1), transcript variant 1
• Database Link:
  Entrez Gene 1289 Human
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Collagen type V is found as short fibrils in skin, bone and placenta. Often it is found in conjunction with other collagen types, especially type I and III.
• Synonyms: COL5A1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion