Apolipoprotein A I (APOA1) Sheep Polyclonal Antibody

CAT#: BP912HRP

Apolipoprotein A I (APOA1) sheep polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (1/1,000 - 1/5,000). Can be used in a two site assay with BM785 bound to the microtitre plate and BP912HRP for detection. Higher titers can be used when TMB is used as the substrate).
  Western blot (1/100 -1/600).
• Reactivities: Human
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Human Apolipoprotein A1
• Specificity: This antibody recognizes Apo-A1 and HDL.
  It does not react with other plasma proteins, including albumin or immunoglobulin fractions. Does not cross react with apolipoprotein B, LDL and VLDL.
• Formulation: PBS containing 50% glycerol, 0.5% BSA and 0.01% Thimerosal.
  Label: HRP
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: HRP
• Storage: Store undiluted at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 335 Human
• Background: Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease.
  The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.
• Synonyms: APOA1, ApoA-I, Apo-AI, ApoAI