Mouse Monoclonal Antibody [Clone ID: OTI6E4]

CAT#: CF190199

Carrier-free (BSA/glycerol-free) IdU mouse monoclonal antibody, clone OTI6E4 (formerly 6E4)

Formulation: Standard Carrier Free


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USD 465.00

In Stock*

Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI6E4
Applications IF, IHC
Recommended Dilution IHC 1:150, IF 1:150
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Iododeoxyuridine coupled to keyhole limpet hemocyanin.
Formulation PBS (pH7.3), 8% Trehalose
Reconstitution Method Reconstitute with PBS (pH7.3). To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids by affinity chromatography
Note 5-chloro-2'-deoxyuridine (ClDU), 5-bromo-2'-deoxyuridine (BrdU), 5-iodo-2'-deoxyuridine (IdU) and 5-ethynyl-2'-deoxyuridine (EdU) are nucleoside analogs of thymidine. Cells that treated with these analogs will incorporate the chemicals into the genomic DNA during S-phase. Immunochemical method detection of these analogs is thus used to quantify the cell proliferation, cell cycle status in vitro or in vivo. Since the thymidine analogs can be passed onto the daughter cells, they can also used to trace dividing cell fate in a short period of time over 3 generations. In addition, combination of different analogs and their specific antibodies can be used to trace cell fate in different time frames.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.