ID2 Mouse Monoclonal Antibody [Clone ID: OTI10C3]

CAT#: CF500183

Carrier-free (BSA/glycerol-free) ID2 mouse monoclonal antibody, clone OTI10C3 (formerly 10C3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID2 (RC205324, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID2. Positive lysates LY400786 (100ug) and LC400786 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ID2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500183)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ID2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500183)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ID2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500183)

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  Immunofluorescent staining of HeLa cells using anti-ID2 mouse monoclonal antibody (TA500183).

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Specifications

Product Data

• Clone Name: OTI10C3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:50
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ID2 (NP_002157) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.7 kDa
• Gene Name: Homo sapiens inhibitor of DNA binding 2 (ID2), mRNA.
• Database Link:
  NP_002157
  Entrez Gene 15902 MouseEntrez Gene 25587 RatEntrez Gene 3398 Human
  UniProt ID Q02363
• Background: The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene.
• Synonyms: bHLHb26; GIG8; ID2A; ID2H

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway