Neurogenin 1 (NEUROG1) Mouse Monoclonal Antibody [Clone ID: OTI5H10]

CAT#: CF500304

Carrier-free (BSA/glycerol-free) NEUROG1 mouse monoclonal antibody, clone OTI5H10 (formerly 5H10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEUROG1 (RC207029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUROG1. Positive lysates LY401855 (100ug) and LC401855 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NEUROG1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500304, Dilution 1:50)

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  Anti-NEUROG1 mouse monoclonal antibody (TA500304) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEUROG1 (RC207029).

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  Immunoprecipitation of NEUROG1 by using TrueMab monoclonal anti-NEUROG1 antibody (Negative control: IP without adding anti-NEUROG1 antibody). For each experiment, 500ul of DDK tagged NEUROG1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEUROG1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Specifications

Product Data

• Clone Name: OTI5H10
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100; IP 2-4ug/mg
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human NEUROG1 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.7 kDa
• Gene Name: Homo sapiens neurogenin 1 (NEUROG1), mRNA.
• Database Link:
  NP_006152
  Entrez Gene 18014 MouseEntrez Gene 29410 RatEntrez Gene 4762 Human
  UniProt ID Q92886
• Synonyms: AKA; bHLHa6; Math4C; NEUROD3; ngn1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors