CD2 Mouse Monoclonal Antibody [Clone ID: OTI4E4]

CAT#: CF500404

Carrier-free (BSA/glycerol-free) CD2 mouse monoclonal antibody, clone OTI4E4 (formerly 4E4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD2 (RC206612, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD2. Positive lysates LY400671 (100ug) and LC400671 (20ug) can be purchased separately from OriGene.

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  Flow cytometric analysis of living 293T cells transfected with CD2 overexpression plasmid (RC206612, Red)/empty vector (PS100001, Blue) using anti-CD2 antibody (TA500404). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500404) (1:500)

Specifications

Product Data

• Clone Name: OTI4E4
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, FC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human CD2 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.4 kDa
• Gene Name: Homo sapiens CD2 molecule (CD2), transcript variant 2, mRNA.
• Database Link:
  NP_001758
  Entrez Gene 914 Human
  UniProt ID P06729
• Background: CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function
• Synonyms: LFA-2; SRBC; T11

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Hematopoietic cell lineage