Melanoma gp100 (PMEL) Mouse Monoclonal Antibody [Clone ID: OTI7B2]

CAT#: CF500423

Carrier-free (BSA/glycerol-free) SILV mouse monoclonal antibody, clone OTI7B2 (formerly 7B2)

Product Images

Anti-SILV mouse monoclonal antibody (TA500423) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SILV (RC200663).

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  Immunofluorescent staining of HT29 cells using anti-SILV mouse monoclonal antibody (TA500423).

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  HEK293T cells transfected with either pCMV6-ENTRY SILV (RC200663) (Red) or empty vector control plasmid (Blue) were immunostained with anti-SILV mouse monoclonal (TA500423, Dilution 1:1,000), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI7B2
• Applications: FC, IF
• Recommended Dilution: IF 1:100, Flow 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SILV (NP_008859) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.3 kDa
• Gene Name: Homo sapiens premelanosome protein (PMEL), transcript variant 3, mRNA.
• Database Link:
  NP_008859
  Entrez Gene 6490 Human
  UniProt ID P40967
• Background: Could be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
• Synonyms: D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV

Reference Data

• Protein Families: Secreted Protein, Transmembrane