Kindlin 2 (FERMT2) Mouse Monoclonal Antibody [Clone ID: OTI25A4]

CAT#: CF500507

Carrier-free (BSA/glycerol-free) FERMT2 mouse monoclonal antibody, clone OTI25A4 (formerly 25A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2. Positive lysates LY416377 (100ug) and LC416377 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and FERMT2-Knockout HeLa cells (KO, Cat# LC812552) were separated by SDS-PAGE and immunoblotted with anti-FERMT2 monoclonal antibody TA500507 (1:500`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500507)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500507)

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  Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (TA500507).

• Immunoprecipitation (IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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Specifications

Product Data

• Clone Name: OTI25A4
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:150, IF 1:100, IP 2ug/500ul
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77.9 kDa
• Gene Name: Homo sapiens fermitin family member 2 (FERMT2), transcript variant 1, mRNA.
• Database Link:
  NP_006823
  Entrez Gene 218952 MouseEntrez Gene 289992 RatEntrez Gene 693958 MonkeyEntrez Gene 10979 Human
  UniProt ID Q96AC1
• Background: Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites
• Synonyms: KIND2; mig-2; MIG2; PLEKHC1; UNC112; UNC112B