Protein kinase Y linked (PRKY) Mouse Monoclonal Antibody [Clone ID: OTI3D11]

CAT#: CF500563

Carrier-free (BSA/glycerol-free) PRKY mouse monoclonal antibody, clone OTI3D11 (formerly 3D11)

Product Images

Anti-PRKY mouse monoclonal antibody (TA500563) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKY (RC210438).

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  HEK293T cells transfected with either pCMV6-ENTRY PRKY (RC210438) (Red) or empty vector control plasmid (Blue) were immunostained with anti-PRKY mouse monoclonal (TA500563, Dilution 1:1,000), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI3D11
• Applications: FC, IF
• Recommended Dilution: IF 1:100, Flow 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRKY (NP_002751) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.7 kDa
• Gene Name: Homo sapiens protein kinase, Y-linked (PRKY)
• Database Link:
  NP_002751
  Entrez Gene 5616 Human
• Background: This gene is similar to the protein kinase, X-linked gene in the pseudoautosomal region of the X chromsoome. The gene is classified as a transcribed pseudogene because it has lost a coding exon that results in all transcripts being candidates for nonsense-mediated decay (NMD) and unlikely to express a protein.Abnormal recombination between this gene and a related gene on chromosome X is a frequent cause of XX males and XY females.
• Synonyms: OTTHUMP00000033227; protein kinase; Y-linked

Reference Data

• Protein Families: Druggable Genome, Protein Kinase